Aggregating images for a spotlight gallery by RegEx and http-Request

Try using the _images operator with the all suffix.

[<current tiddler>_images:all[]]

With this suffix the operator wikifies the tiddler and attempts to extract all images.

I assume your folder is being served by a HTTP server? If it provides a directly listing that could be fetched and parsed. Otherwise you need some code on the server that can respond to an HTTP request and return a list of images.